Our project lays the foundation for a new scientific collaboration among urban ecologists, ecosystem scientists, and evolutionary biologists to achieve deep integration of knowledge, techniques, and expertise to understand urban eco-evolutionary dynamics. Our aim is to create a community of scientists bringing together scientists from diverse disciplines studying a variety of study systems across diverse US and international institutions and establishing a new collaboration with the ecosystem science community through two urban (Baltimore and Phoenix) and one non-urban (Sevilleta) long term ecological research (LTER) sites.